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Applications of BBa_K861074

In our experiment, cells that expressed protein which was nothing to do with cellulose synthesis was used as a control.We transformed the seven genes involved in cellulose synthesis into E.coli and meaaured cellulose production. In the step of measuring total cellulose production, exceed cellulase was appended and incubated at 50 ℃ for 1 hour, and results show that the overexpression of adrA can help increase the ability of cellulose synthesis.

And also we co-overexpress adrA with bcsA. Cellulose output in both supernatant and deposit were show in the following figure. The cellulose production in adrA+bcsA is more than 0.6 mg/mL, which is almost two times higher than that of control.

All these results indicated that AdrA can increase the ability of cellulose synthesis

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