Part:BBa_K902041:Experience

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Applications of BBa_K902041

amdA is an Amidase gene responsible for the removal of primary amine's from a variety of compounds. We validated the ability of this gene to remove the primary amine group from benzamine. This was evaluated through GC/MS analysis and detailed on our <a href="http://2012.igem.org/Team:Calgary/Notebook/Protocols/AmidaseAssay">protocol page</a>. This is detailed in the figures below:

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Figure 1: GC chromatograph demonstrating the degradation of benzamide by AmdA compared to an E. coli control. Additionally, a smaller peak with an earlier retention time is produced solely in the sample run.

Figure 2: Mass Spectra for the peak at a retention time of 10.5 minutes from Figure 1 representing the substrate benzamine. Additionally, the peak at retention time of 9 minutes represents benzoic acid based on its mass suggesting that their is a small accumulation of product.

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