Reporter

Part:BBa_K883705

Designed by: Lisa Sonnenberg   Group: iGEM12_Wageningen_UR   (2012-10-26)
Revision as of 07:42, 26 October 2012 by Registry (Talk | contribs)

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IPTG induced promoter + GFP with a His-tag fused to a coiled coil

GFP with His tag at the C-terminus and the E-coil at the N-terminus behind an IPTG induced promoter to use it as reporter for the E- and K-coil Plug and Apply system

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 233
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 233
    Illegal NotI site found at 239
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 233
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 233
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 233
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1041


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Parameters
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