Device

Part:BBa_K883401

Designed by: Marnix Vlot   Group: iGEM12_Wageningen_UR   (2012-10-25)
Revision as of 13:54, 25 October 2012 by Registry (Talk | contribs)

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PLRV Coat Protein gene under IPTG induced promotor

This part consists of BBa_J04500 (IPTG inducible promotor + RBS) and the coding sequence for the major coat protein of the Potato Leafroll Virus (PLRV). This sequence was isolated from a PLRV-infected Agria potato leaf by iGEM team Wageningen UR 2012. This was done by cDNA synthesis followed by PCR. The PLRV major Coat Protein should be capable of forming a Virus-Like Particle, but this was not (yet) confirmed for this part. This gene was isolated from potato leaf roll virus (PLRV) infected plants. The obtained sequence of the coding gene is 100% identical to several PLRV isolates available in the NCBI database. Expression of a similar viral coat proteins was performed by the iGEM2012 team of Wageningen UR, strongly suggesting that this part can be expressed in E. coli as well. In the figure below, the predicted 3 dimensional structure can be seen. The coat proteins will form a icosahedral shaped Virus Like Particle (VLP) with a radius of about 11,5 nm composed of 180 coat proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 806
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//chassis/prokaryote/ecoli
//direction/forward
//promoter
Parameters
biologyPotato Leafroll Virus
proteinMajor Coat Protein