Generator

Part:BBa_K801076

Designed by: Dennis Hell   Group: iGEM12_TU_Munich   (2012-09-21)
Revision as of 19:12, 20 October 2012 by Nadine1990 (Talk | contribs)

Yeast expression cassette for CaXMT1 and CaMXMT1

Yeast Expression Cassette for the enzymes 1 and 2 of the caffeine biosynthesis pathway

Expression of CaXMT1 is controlled by the yeast TEF2 promoter and the yeast ADH1 terminator.

Expression of CaMXMT1 is controlled by the yeast TEF1 promoter and the yeast ADH1 terminator.

This BioBrick is a generator for the first two enzymes envolved in caffeine biosynthesis: methylxanthosine N-methyltransferase 1 and 7- methylxanthine N-methyltransferase 1. It is made up of the two single generators for CaXMT1 and CaMXMT1, which means that CaXMT1 is regulated by the Tef2 promoter and CaMXMT1 is regulated by the Tef1 promoter. In both cases, the Adh1 terminator was used.

Background and principles

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3613
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 138
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2385


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Categories
Parameters
None