Part:BBa_K814005

shinorine biosynthetic pathway

Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. DHQS catalyzes the first step in the pathway, converting sedoheptulose-7-phosphate into dehydroquinate. Dehydroquinate-O-methyltransferase (O-MT) then generates 4-deoxygadusol, which is converted to mycosprine-glycine by ATP-grasp (ATPG). Finally, mycosporine-glycine is converted into shinorine by a non-ribosomal peptide synthase (NRPS).

This composite part contains protein generators for DHQS, O-MT, ATPG and the shinorine NRPS.

Figure 1. The shinorine biosynthetic pathway.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 250
  Illegal EcoRI site found at 2397
  Illegal XbaI site found at 3468
  Illegal XbaI site found at 6853
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 250
  Illegal EcoRI site found at 2397
  Illegal NotI site found at 1373
  Illegal NotI site found at 2970
  Illegal NotI site found at 4030
  Illegal NotI site found at 6917
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 250
  Illegal EcoRI site found at 2397
  Illegal BglII site found at 134
  Illegal BglII site found at 1587
  Illegal BglII site found at 3184
  Illegal BglII site found at 4244
  Illegal XhoI site found at 1381
  Illegal XhoI site found at 2978
  Illegal XhoI site found at 4038
  Illegal XhoI site found at 6925
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 250
  Illegal EcoRI site found at 2397
  Illegal XbaI site found at 3468
  Illegal XbaI site found at 6853
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 250
  Illegal EcoRI site found at 2397
  Illegal XbaI site found at 3468
  Illegal XbaI site found at 6853
  Illegal AgeI site found at 3568
  
• 1000
  COMPATIBLE WITH RFC[1000]