Inverter

Part:BBa_K783067

Designed by: Traci Haddock   Group: iGEM12_BostonU   (2012-10-02)
Revision as of 01:42, 10 October 2012 by THaddock (Talk | contribs)

pBad-pTetR inverter

This is an inverter with pBad driving tetR with GFP as a reporter. pTetR has RFP has a reporter. We used a weaker RBS (B0032) to control the tetR expression and it helped the inverter function properly.

We used flow cytometry to measure the function of our inverter.

Inverter.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1247
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1187
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 3490
    Illegal AgeI site found at 3602
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2634
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None