Inverter

Part:BBa_K783067

Designed by: Traci Haddock   Group: iGEM12_BostonU   (2012-10-02)
Revision as of 01:41, 10 October 2012 by THaddock (Talk | contribs)

pBad-pTetR inverter

This is an inverter with pBad driving tetR with GFP as a reporter. pTetR has RFP has a reporter. We used a weaker RBS (B0032) to control the tetR expression and it helped the inverter function properly.

Inverter.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1247
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1187
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 3490
    Illegal AgeI site found at 3602
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2634
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None