Part:BBa_K783083

mmoR from M. trichosporium OB3b MoClo format

mmoR is a sigma-54 regulatory activator amplified from Methylosinus trichosporium OB3b and positively regulates pMmoR (BBa_K783066). Copper represses that activity.

This has two MoClo fusion sites flanking the part: 5' C [part] D 3'

The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Note: This gene has three PstI cut sites, so this cannot be built using standard BioBrick protocols.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1130
  Illegal PstI site found at 1406
  Illegal PstI site found at 2039
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 1130
  Illegal PstI site found at 1406
  Illegal PstI site found at 2039
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 35
  Illegal XhoI site found at 407
  Illegal XhoI site found at 1883
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1130
  Illegal PstI site found at 1406
  Illegal PstI site found at 2039
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1130
  Illegal PstI site found at 1406
  Illegal PstI site found at 2039
  Illegal NgoMIV site found at 80
  Illegal NgoMIV site found at 262
  Illegal NgoMIV site found at 508
  Illegal NgoMIV site found at 776
  Illegal NgoMIV site found at 860
  Illegal NgoMIV site found at 1197
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1103