Regulatory

Part:BBa_K783082

Designed by: Traci Haddock   Group: iGEM12_BostonU   (2012-10-03)
Revision as of 18:54, 7 October 2012 by THaddock (Talk | contribs)

pMmoR, a sigma 54-regulated promoter MoClo format

pMmoR is a promoter isolated from Methylosinus trichosporium OB3b and is in the sigma-54 regulatory family of promoters. The gene mmoR from Methylosinus trichosporium OB3b induces pMmoR and copper represses that activity.

This has two MoClo fusion sites flanking the part: 5' C [part] D 3'

The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/prokaryote/ecoli/sigma54
Parameters
negative_regulatorsCopper
originMethylosinus trichosporium OB3b
positive_regulatorsmmoR BBa_K783083