Regulatory
Part:BBa_K783082
Designed by: Traci Haddock Group: iGEM12_BostonU (2012-10-03)
pMmoR, a sigma 54-regulated promoter MoClo format
pMmoR is a promoter isolated from Methylosinus trichosporium OB3b and is in the sigma-54 regulatory family of promoters. The gene mmoR from Methylosinus trichosporium OB3b induces pMmoR and copper represses that activity.
This has two MoClo fusion sites flanking the part: 5' C [part] D 3'
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//rnap/prokaryote/ecoli/sigma54
negative_regulators | Copper |
origin | Methylosinus trichosporium OB3b |
positive_regulators | mmoR BBa_K783083 |