Part:BBa_K592009:Experience

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UNIQc6dd370334bfd18d-partinfo-00000000-QINU UNIQc6dd370334bfd18d-partinfo-00000001-QINU

BBa_K592009 iGEM Groningen 2012

Our team has managed to couple this biobrick part with our promoters: alsT, fnr, and sboA. The cloning was done in BBa_K818000 (plasmid backbone for B. subtilis, engineered by team Groningen 2012), to allow color expression in B. subtilis. We utilized a strong RBS BBa_B0034 for pigment expression in E. coli and B. subtilis. The purple/blue colour was strongly visible in E.coli without any induction, while the expression in B. subtilis was more subtle (B. subtilis colony looks slightly blue on the plate agar). After induction of the promoter before AmilCP, B. subtilis also turned clearly purple/blue. Please have a look at the page of BBa_K818400 for more information.

No review score entered. User:agynna

iGEM Team Uppsala University 2012 We have observed that expression of amilCP confers a noticeable fitness cost in E coli. This is surprising, given that such behaviour is not seen in other homologous chromoproteins and fluorescent proteins. We thus recommend using the new aeBlue (BBa_K864401) chromoprotein for blue color expression, which also has a more clear blue color.

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