Device

Part:BBa_K935005:Experience

Designed by: Twaddle Lab   Group: iGEM12_IvyTech-South_Bend   (2012-10-02)
Revision as of 15:39, 6 October 2012 by Geotwaddle (Talk | contribs) (→‎Applications of BBa_K935005)

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We transformed E.coli with a series of our arsenic responsive constructs. BBa_K935005 contains three ArsR genes under the control of the pArs promoter. We found it sensitivity to 6-fold less than that BBa_K935002, which contains only one ArsR gene under the control of pArs and essentially the same as that of BBa_K935004, which contains two ArsR genes. In addition the magnitude of the response was reduced 10-fold less at 100uM arsenate compared to that of BBa-935002.

BBa K935004 and BBa K935005 Response to Arsenate.jpg

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