Device
2gen:ArsR

Part:BBa_K935004:Experience

Designed by: Twaddle Lab   Group: iGEM12_IvyTech-South_Bend   (2012-10-02)
Revision as of 15:37, 6 October 2012 by Geotwaddle (Talk | contribs) (Applications of BBa_K935004)

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We transformed E.coli with a series of our arsenic responsive constructs. BBa_K935004 contains two ArsR genes under the control of the pArs promoter. We found it sensitivity to 6-fold less than that BBa_K935002, which contains only one ArsR gene under the control of pArs. In addition the magnitude of the response was reduced 10-fold less at 100uM arsenate compared to that of BBa-935002.

BBa K935004 and BBa K935005 Response to Arsenate.jpg

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