Device

Part:BBa_K769003:Design

Designed by: Genki Sakai   Group: iGEM12_Tokyo-NoKoGen   (2012-09-19)
Revision as of 07:24, 6 October 2012 by G.Sakai (Talk | contribs) (→‎Design Notes)

Pconst.(High)-RBS-NpSRII-9a.a.linker_NpHtrII-EnvZ-Double terminator-PompR-RBS-GFP-Double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2626
    Illegal XhoI site found at 2527
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 134
    Illegal NgoMIV site found at 392
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1088
    Illegal SapI site found at 862


Design Notes

[http://2012.igem.org/Team:Tokyo-NoKoGen/Project/rhodopsin]

Source

Halobacteria

References