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Part:BBa_K811000

Designed by: Avin Veerakumar   Group: iGEM12_Penn   (2012-10-03)
Revision as of 01:03, 6 October 2012 by Qiaop (Talk | contribs)

Cytolysin A (ClyA)

Cytolysin A (ClyA) pore forming protein causes cell lysis. After cloning into expression vector, clyA expression and activity can be visualized on 5% Sheep's Blood Agar plates. Expected transcript size: 34kDa


Usage and Biology

ClyA is a protein native to E. coli, Shigella flexneri, and Salmonella typhi that is capable of forming 13-mer pore complexes in a redox-independent manner. Expression of clyA in the absence of other hemolytic toxins is sufficient to induce hemolysis experimentally, and is therefore considered to be a potent cytolytic agent. Unlike a similar protein, HlyA, ClyA is not synthesized as a protoxin, which requires further posttranslational modifications to become active. ClyA functional immediately following translation of mRNA to protein. In vitro and i n vivo,

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None