Reporter

Part:BBa_K817013

Designed by: Anjui Wu   Group: iGEM12_NTU-Taida   (2012-09-23)
Revision as of 12:01, 5 October 2012 by Anjui (Talk | contribs)

pCI(OR1-OL1)-mRFP

As we sought to design a thermal sensitive composite part, we integrate our modified pCI promoter(modification of pCI, BBa_R0051) with temperature sensitive CI repressor, which is cloned from Harvard’s thermal regulation part, 2008. (BBa_K098988). We showed the modified promoter design had superior repression effects.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 625
    Illegal AgeI site found at 737
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/apCI(OR1-OL1)-mRFP