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Part:BBa_K900000:Design

Designed by: Austin Jones   Group: iGEM12_Berkeley   (2012-09-27)
Revision as of 15:00, 4 October 2012 by Wideloache (Talk | contribs) (→‎Source)

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PAmCherry (photoactivatable red fluorescent protein)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 682
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene was codon optimized for yeast and internal restriction sites were removed for compatibility with the 2012 Berkeley iGEM team's Golden Gate cloning scheme.

Source

Synthesized in house. Coding sequence was obtained from an engineered version of mCherry, documented in Subach et al., 2009. Of the multiple versions of PAmCherry described in the paper, we synthesized PAmCherry2.

References

Subach, F. V. et al. Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. Nature Methods 6, 153–159 (2009).