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Part:BBa_K900000:Design

Designed by: Austin Jones   Group: iGEM12_Berkeley   (2012-09-27)
Revision as of 14:57, 4 October 2012 by Wideloache (Talk | contribs) (→‎Design Notes)

PAmCherry (photoactivatable red fluorescent protein)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 682
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene was codon optimized for yeast and internal restriction sites were removed for compatibility with the 2012 Berkeley iGEM team's Golden Gate cloning scheme.

Source

Synthesized in house. Previously engineered version of RFP from Subach et al., 2009.

References

Subach, F. V. et al. Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. Nature Methods 6, 153–159 (2009).