Composite

Part:BBa_K769018

Designed by: Mayumi Nakamura   Group: iGEM12_Tokyo-NoKoGen   (2012-09-19)
Revision as of 07:12, 4 October 2012 by Miyake (Talk | contribs)

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Promoter(Pbad)-RBS-luxD-DT-Promoter(high)-RBS-lux CABFEG-DT

Promoter(Pbad)-RBS-luxD-DT-Promoter(high)-RBS-lux CDBFEG-DT (lux operon from Photobacterium phosphoreum NCMB844)


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This part is constructed from our cloned lux operon, BBa_K769010.
It consists of an inducible luxD gene and constitutively epxressed luxCABEFG gene. We wanted to make the bioluminescent reaction time faster than BBa_K769011. By constitutively expresseing all lux gene except the luxD gene, we thought that the induction of just one gene would shorten the reaction time to observe bioluminescent.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 2309
    Illegal NheI site found at 2332
    Illegal NheI site found at 2807
    Illegal NheI site found at 6149
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6611
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 3961
    Illegal XhoI site found at 4469
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 3092
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2986
    Illegal BsaI.rc site found at 3668
    Illegal SapI site found at 961


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Categories
Parameters
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