Composite
Part:BBa_K769018
Designed by: Mayumi Nakamura Group: iGEM12_Tokyo-NoKoGen (2012-09-19)
Promoter(Pbad)-RBS-luxD-DT-Promoter(high)-RBS-lux CABFEG-DT
Promoter(Pbad)-RBS-luxD-DT-Promoter(high)-RBS-lux CDBFEG-DT (lux operon from Photobacterium phosphoreum NCMB844)
This part is constructed from our cloned lux operon, BBa_K769010.
It consists of an inducible luxD gene and constitutively epxressed luxCABEFG gene. We wanted to make the bioluminescent reaction time faster than BBa_K769011. By constitutively expresseing all lux gene except the luxD gene, we thought that the induction of just one gene would shorten the reaction time to observe bioluminescent.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
Illegal NheI site found at 2309
Illegal NheI site found at 2332
Illegal NheI site found at 2807
Illegal NheI site found at 6149 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 6611
Illegal BamHI site found at 1144
Illegal XhoI site found at 3961
Illegal XhoI site found at 4469 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 3092 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2986
Illegal BsaI.rc site found at 3668
Illegal SapI site found at 961
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Categories
Parameters
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