Composite

Part:BBa_K769015

Designed by: Mayumi Nakamura   Group: iGEM12_Tokyo-NoKoGen   (2012-09-19)
Revision as of 07:06, 4 October 2012 by Miyake (Talk | contribs)

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Promoter(high)-RBS-lux CDBFEG-DT

Promoter(high)-RBS-lux CDBFEG-DT (lux operon from Photobacterium phosphoreum NCMB844)
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This part is constructed from our cloned lux operon, BBa_K769010.
The luxCDBEFG is missing a luxA gene. Our aim was to make the bioluminescent reaction time faster than BBa_K769011, which is a construct where the whole lux system, luxCDABEFG is expressed under an arabinose inducible promoter. This is an intermediate of such device for shortening the reaction time. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 505
    Illegal NheI site found at 3669
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4131
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 790
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 684
    Illegal BsaI.rc site found at 1366


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