Coding
Part:BBa_K804013:Experience
Designed by: Joe Ho Group: iGEM12_British_Columbia (2012-10-02)
Revision as of 04:03, 4 October 2012 by Jacobtoth (Talk | contribs) (→MetA Knockout Complementation by British Columbia iGEM 2012)
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MetA Knockout Complementation by British Columbia iGEM 2012
This part consists of an rhamnose promoter, a strong RBS, and the MetA coding gene. It is unable to complement a MetA knockout, and the growth rate appears to unaffected by the amount of rhamnose that is added. The data for the Rhamnose Inducible TrpA Coding Gene is also shown on this graph.
To generate this graph we cultured an E. coli MetA knockout transformed with this part in M9 minimal media with the indicated concentrations of rhamnose, and measured the OD600 every 15 minutes.