Coding

Part:BBa_K902062:Design

Designed by: Tsarina VanWieren   Group: iGEM12_Calgary   (2012-10-02)
Revision as of 03:40, 4 October 2012 by Tsarina.VanWieren (Talk | contribs) (→‎Design Notes)

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CAR (Nocardia carboxylic acid reductase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1302
    Illegal NotI site found at 1691
    Illegal NotI site found at 2452
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1016
    Illegal BglII site found at 1448
    Illegal BglII site found at 1619
    Illegal BglII site found at 2102
    Illegal XhoI site found at 1498
    Illegal XhoI site found at 1582
    Illegal XhoI site found at 2509
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3108
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 256
    Illegal BsaI.rc site found at 1402
    Illegal BsaI.rc site found at 1798


Design Notes

The original CAR gene contained six illegal cut sites for BioBrick BB-2 RFC[12]: three NotI sites, two PstI sites, and one EcoRI sites. The two PstI sites and one EcoRI site were mutagenized (see features). However, three NotI sites remain.

Source

Nocardia iowensis strain NRRL 5646

References

He A, Li T, Daniels L, Fotheringham I, Rosazza J.P.N. Nocardia sp. Carboxylic Acid Reductase: Cloning, Expression, and Characterization of a New Aldehyde Oxidoreductase Family. Applied and Environmental Microbiology 2004 Mar;70(3):1874–1881.