Generator

Part:BBa_K902027:Design

Designed by: Somshukla Chaudhuri   Group: iGEM12_Calgary   (2012-10-02)
Revision as of 03:20, 4 October 2012 by Rai (Talk | contribs) (→‎Design Notes)

LacI(R0010)-MOCO riboswitch-CviAII


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 471
    Illegal BglII site found at 727
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This brick was constructed using the biobrick assembly method. There are no illegal cut sites.

Source

The CviaII and the Moco riboswtich were synthesized through IDT. The sequence was derived from the NCBI Gene Database.

References

1) Regulski EE, Moy RH, Weinberg Z, Barrick JE, Yao Z, Ruzzo WL, Breaker RR. A widespread riboswitch candidate that controls bacterial genes involved in molybdenum cofactor and tungsten cofactor metabolism.Mol Microbiol. 2008 May; 68(4): 918–932

2) Xia Y, Burbank DE, Van Etten JL. Restriction endonuclease activity induced by NC-1A virus infection of a Chlorella-like green alga. Nucl. Acids Res. (1986) 14 (15): 6017-6030.