Composite

Part:BBa_K902028:Design

Designed by: Somshukla Chaudhuri   Group: iGEM12_Calgary   (2012-10-02)
Revision as of 03:16, 4 October 2012 by Rai (Talk | contribs) (→‎References)

Moco Riboswitch-CviAII


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 263
    Illegal BglII site found at 519
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There are no illegal cut sites. This part was constructed with the Moco riboswitch and the CviaII restriction endonuclease using the BioBrick Assembly method.

Source

The Moco-CviaII composite part.

References

1) Regulski EE, Moy RH, Weinberg Z, Barrick JE, Yao Z, Ruzzo WL, Breaker RR. A widespread riboswitch candidate that controls bacterial genes involved in molybdenum cofactor and tungsten cofactor metabolism.Mol Microbiol. 2008 May; 68(4): 918–932

2) Xia Y, Burbank DE, Van Etten JL. Restriction endonuclease activity induced by NC-1A virus infection of a Chlorella-like green alga. Nucl. Acids Res. (1986) 14 (15): 6017-6030.