Translational_Unit

Part:BBa_K902056:Design

Designed by: Lisa Oberding, Ali Honarmand   Group: iGEM12_Calgary   (2012-10-02)
Revision as of 03:07, 4 October 2012 by Lisa.O (Talk | contribs) (→‎References)

dszC behind an RBS (B0034)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 96
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 900
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1050


Design Notes

Mutagenesis


Source

Amplified from a Rhodococcus environmental isolate via PCR.

References

Xi L, Squires CH, Monticello DJ, Childs JD. A flavin reductase stimulates DszA and DszC proteins of Rhodococcus erythropolis IGTS8 in vitro. Biochem Biophys Res Commun. 1997 Jan 3;230(1):73-5.