Translational_Unit

Part:BBa_K902051:Design

Designed by: Lisa Oberding, Ali Honarmand   Group: iGEM12_Calgary   (2012-10-01)
Revision as of 02:55, 4 October 2012 by Lisa.O (Talk | contribs) (→‎References)

dszA behind and RBS (B0034)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 772
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 61
    Illegal NgoMIV site found at 276
    Illegal NgoMIV site found at 580
    Illegal NgoMIV site found at 700
    Illegal AgeI site found at 235
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1084


Design Notes

See DszA mutations


Source

Amplified from an environmental isolate of R. erythropolis

References

Gou,Z., Li,X., Xing,J., Liu,H. and Chen,J. LSSE8-1 and its desulfurizational genes. Laboratory of Separation Science and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 1# Beiertiao, Zhongguancun, Haidian, Beijing 100080, China. 2003.