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Composite

Part:BBa_K781006

Designed by: Kevin Chen   Group: iGEM12_Queens_Canada   (2012-10-03)
Revision as of 02:53, 4 October 2012 by Chev1n (Talk | contribs)

[R0010][B0034] - RFP-FliC Deletion Chimera

This is the modified RFP protein obtained from J04450, that has been incorporated into the E. coli flagellin as a deletion.

This part can be used as a template for making other PCR overlap insertions. After transforming the product, and digesting the template plasmid with Dpn1, any template that has not been successfully digested will form red colonies.

Please refer to the page for K781001 for characterization results until this page is updated.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1884
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 529
    Illegal AgeI site found at 1362
    Illegal AgeI site found at 1474
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
n/a[R0010][B0034] - RFP-FliC Deletion Chimera