Generator
Part:BBa_K902005:Design
Designed by: Emily Hicks Group: iGEM12_Calgary (2012-08-29)
Beta-D-glucoside glucohydrolase (bglX) constitutive generator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1701
Illegal AgeI site found at 1923
Illegal AgeI site found at 2112 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
An illegal pstI site at the 15th and 16th amino acid position was removed vis site-directed mutagenesis.
Source
The beta-D-glucoside glucohydrolase (bglX) gene was amplified from the genome of TOP10 E. coli via polymerase chain reaction.
References
Yang M, Luoh SM, Goddard A, Reilly D, Henzel W, Bass S.The bglX gene located at 47.8 min on the Escherichia coli chromosome encodes a periplasmic beta-glucosidase.Microbiology. 1996 Jul;142 ( Pt 7):1659-65.