Coding
Part:BBa_K898890:Design
Designed by: Hsiao-Han Chen. Anthony Chau. Uros Kuzmanovic. Zefeng Wang. Group: iGEM12_UIUC_Illinois (2012-09-30)
wtPUF+PIN
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1297
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 35
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Source
The PUF domain from human Pum1 (residue 828-1176) and the PIN domain from human Smg6 (residue 1238-1421)