Coding

Part:BBa_K936030

Designed by: Sergio Castro Waqued   Group: iGEM12_UC_Davis   (2012-10-03)
Revision as of 02:25, 4 October 2012 by Ckdeniston (Talk | contribs)

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LC Cutinase mutant T96A

Mutant of the Polyetheylene Terephtalate degrading protein LC Cutinase (Bba_K936000). The threonine at the 96th amino acid position is changed to an alanine. This part was created using site directed mutagenesis to change an "ACG" codon to a "GCA" codon.
UC_Davis_First_pNPB_Run.png
(Above Graph) Results of pNPB assay detailing the constitutive construct as having the highest esterase activity.UC_Davis_Third_pNPB_Run.png
(Above Graphs) The graphs above detail higher activity among the constitutive construct and some mutant variants in esterase activity compared to the control and wild type per unit cell.
UC_Davis_Last_pNPB_Run.png
(Above graph) This graph represents a possible production of cutinase by the cells upon entering stationary phase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 600
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None