Composite

Part:BBa_K936021

Designed by: Mattan Hamou   Group: iGEM12_UC_Davis   (2012-10-01)
Revision as of 02:23, 4 October 2012 by Ckdeniston (Talk | contribs)

Cutinase and polyhistidine tag

LC Cutinase coding sequence with polyhistidine tag attached. The polyhistidine tag allows our research team to perform assays to discover where our enzyme is being released, both in the media and in the cell.
UC_Davis_First_pNPB_Run.png
(Above Graph) Results of pNPB assay detailing the constitutive construct as having the highest esterase activity.UC_Davis_Third_pNPB_Run.png
(Above Graphs) The graphs above detail higher activity among the constitutive construct and some mutant variants in esterase activity compared to the control and wild type per unit cell.
UC_Davis_Last_pNPB_Run.png
(Above graph) This graph represents a possible production of cutinase by the cells upon entering stationary phase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 600
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None