Part:BBa_K847080:Experience

Assay
Liquid cultures of negative control, recA, dps, and sdaB transformed NEB5α E. coli were grown up over night at 37°C. After incubation, LB + amp media of pH 3.54, 4.60, 6.93, 9.07, 9.50 were created using HCl and NaOH. In a 96 well plate, 8 wells were filled with LB + amp blank. Next, 5uL of each transformed bacteria was placed in five different pH wells. This was replicated four times, thus, creating a 96 well plate with 8 blanks, and four replicates of each test construct at five different pH’s. Finally, the OD600 was measured every five minutes for 24 hours while the plate was incubated at 37°C, shook for 1 minute before every sample and between every sample in a spectrophotometer.

Results
Works. Refer to: http://2012.igem.org/Team:Stanford-Brown/HellCell/pH

Applications of BBa_K847080

Astrobiology revolves around three central questions: "Where do we come from?", "Where are we going?", and "Are we alone?" To approach the second question, the Hell Cell subgroup of the Stanford-Brown iGEM team developed BioBricks that allow a cell to survive harsh extraterrestrial conditions. Such a toolset could create a space-ready synthetic organism to perform useful functions off-world. This gene is one of the toolset, potentially conferring resistance to basic conditions to otherwise intolerant bacteria.

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