Regulatory
pRha

Part:BBa_K902065:Design

Designed by: Himika Dastidar, Chris Wintersinger   Group: iGEM12_Calgary   (2012-10-02)
Revision as of 02:22, 4 October 2012 by Jeffaddison89 (Talk | contribs)

Rhamnose inducible, glucose repressible promoter (pRha)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

This promoter was commercially synthesized from the sequence characterized by Jeske and Altenbuchner (2010).

References

Egan, S. M., Schleif, R. F., & others. (1993). A regulatory cascade in the induction of rhaBAD. Journal of Molecular Biology, 234(1), 87-98.

Jeske, M., & Altenbuchner, J. (2010). The escherichia coli rhamnose promoter rhaP BAD is in pseudomonas putida KT2440 independent of crp--cAMP activation. Applied Microbiology and Biotechnology, 85(6), 1923-1933.