Coding

Part:BBa_K902024:Design

Designed by: Somshukla Chaudhuri   Group: iGEM12_Calgary   (2012-10-02)
Revision as of 02:15, 4 October 2012 by Rai (Talk | contribs) (→‎Source)

moa Operon (MoaABCDE)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 539
    Illegal BamHI site found at 665
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 952
    Illegal AgeI site found at 1851
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 603
    Illegal SapI.rc site found at 1964


Design Notes

moa operon


Source

The moaABCDE operon was first seen to be used in conjunction with the Moco riboswitch in the paper cited in the reference sequence. The sequence was derived from Profiling of E.Coli Chromosome (PEC) and NCBI Gene Database.

References

1) Regulski EE, Moy RH, Weinberg Z, Barrick JE, Yao Z, Ruzzo WL, Breaker RR. A widespread riboswitch candidate that controls bacterial genes involved in molybdenum cofactor and tungsten cofactor metabolism.Mol Microbiol. 2008 May; 68(4): 918–932