Coding
otsB

Part:BBa_K847061:Design

Designed by: Rashmi Sharma, Vishesh Jain, Kendrick Wang   Group: iGEM12_Stanford-Brown   (2012-08-09)
Revision as of 02:01, 4 October 2012 by Rsharma (Talk | contribs) (→‎References)

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''Escherichia coli'' osmoregulatory trehalose synthesis B (otsB)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 455
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was isolated via colony PCR from Escherichia coli. It does not contain promoter, RBS, transcriptional terminator, etc. These elements must be connected to this part for proper functionality.

Source

Escherichia coli

References

Calahan, D., Dunham, M., DeSevo, C., Koshland, D.E. (2011). Genetic analysis of desiccation tolerance in Saccharomyces cerevisiae. Genetics, 189: 507-519.

Bonaterra, A., Camps, J., Montesinos, E. (2005). Osmotically induced trehalose and glycine betaine accumulation improves tolerance to desiccation, survival and efficacy of the postharvest biocontrol agent Pantoea agglomerans EPS125. FEMS Microbiol. Lett., 250: 1-8.