Generator

Part:BBa_K902002:Design

Designed by: Emily Hicks   Group: iGEM12_Calgary   (2012-08-29)
Revision as of 01:55, 4 October 2012 by Lisa.O (Talk | contribs) (→‎References)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Beta-glucuronidase (uidA) inducible generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 732
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

The beta-glucuronidase (uidA) gene was amplified from the genome of E. coli using polymerase chain reaction.

References

Jefferson RA, Burgess SM, Hirsh D. beta-Glucuronidase from Escherichia coli as a gene-fusion marker. Proc Natl Acad Sci U S A. 1986 Nov;83(22):8447-51.