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Part:BBa_K900001:Design

Designed by: Austin Jones   Group: iGEM12_Berkeley   (2012-09-27)
Revision as of 01:36, 4 October 2012 by Robertchen1992 (Talk | contribs) (→‎Source)

PAGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization for yeast and internal restriction site removal to be compatible with our Golden Gate cloning scheme.


Source

Synthesized in house and cloned into Addgene plasmid [http://www.addgene.org/11911/ l11911] Previously engineered version of RFP from Patterson and Lippincott-Schwartz, 2002.

References

Patterson, G. H. & Lippincott-Schwartz, J. A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells. Science 297, 1873–1877 (2002).