Regulatory

Part:BBa_K844010

Designed by: Kathleen Miller   Group: iGEM12_Utah_State   (2012-10-03)
Revision as of 01:27, 4 October 2012 by Skiracer96 (Talk | contribs)

Enhanced tRNA Promoter for ''E. coli''

Usage and Biology

This tRNA terminator seqeuence is based around the downstream sequence of the E. coli Ala(GCC) tRNA. It contains the region of 3-6 T’s that is characteristic of tRNA terminators and an additional 10 bp of native sequence. This additional region of native sequence is to ensure RNA termination and to act as a small spacer region between tRNA genes.

It is important to use a tRNA terminator when expressing tRNA parts, as rho-dependent and rho-independent promoters rely on additional RNA sequence after the tRNA gene to function, and the addition of more RNA bases on a tRNA can severely affect its ability to function.


Related Parts

BBa_K8440011 - This part is a tRNA specific terminator for E. coli


References

Bauer B.F., Elford R.M., and Holmes W.M. 1993. Mutagenesis and functional analysis of the Escherichia coli tRNA (1Leu) promoter. Mol microbio. 7:265–273.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None