Coding

Part:BBa_K842004

Designed by: Sean P. Curran, Percy Genyk, Ellen Park, Rebecca, Gao, Stephan Genyk, Megan Bernstein, Rachel Kohan, Eric Siryj, Luke Quinto   Group: iGEM12_USC   (2012-10-02)
Revision as of 01:08, 4 October 2012 by Rkohan (Talk | contribs)

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fliC

USC flic.gif

Transcription of fliC generates the flagellin protein, which is then exported to form the flagella filament. This structure is the backbone of the flagella.

Used for reconstitution of flagella apparatus in E. coli B strains.

Cloning and Uses: This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used in conjunction with the entire flagella synthesis genetic pathway to reproduce a complete flagella apparatus.














Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 547
    Illegal PstI site found at 872
    Illegal PstI site found at 919
    Illegal PstI site found at 1486
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 547
    Illegal PstI site found at 872
    Illegal PstI site found at 919
    Illegal PstI site found at 1486
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1224
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 547
    Illegal PstI site found at 872
    Illegal PstI site found at 919
    Illegal PstI site found at 1486
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 547
    Illegal PstI site found at 872
    Illegal PstI site found at 919
    Illegal PstI site found at 1486
    Illegal AgeI site found at 301
    Illegal AgeI site found at 709
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None