Coding
SodMn

Part:BBa_K847003:Design

Designed by: Rashmi Sharma, Vishesh Jain, Kendrick Wang   Group: iGEM12_Stanford-Brown   (2012-08-09)
Revision as of 23:44, 3 October 2012 by Rsharma (Talk | contribs) (→‎References)

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''Deinococcus radiodurans'' Superoxide dismutase, SodMn


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 139
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 139
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 139
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 139
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 139
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was isolated via colony PCR from Deinococcus radiodurans. It does not contain promoter, RBS, transcriptional terminator, etc. These elements must be connected to this part for proper functionality.

Source

Deinococcus radiodurans

References

Daly, M.J. (2009) A new perspective on radiation resistance based on Deinococcus radiodurans. Nature Rev. Microbiol, 7: 237-245.

Slade, D. and Miroslav, R. (2011). Oxidative stress resistance in Deinococcus radiodurans. Microbiol. Mol. Biol. Rev., 75(1): 133-191.

Gao, N., Ma, B., Zhang, Y., Song, Q., Chen, L., Zhong H. (2009). Gene expression analysis of four radiation-resistant bacteria. Genomics Insights, 2, 11-22.