Tag
His

Part:BBa_K112703:Experience

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-10-21)
Revision as of 22:49, 3 October 2012 by Catramp (Talk | contribs) (→‎User Reviews)

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This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K112703

User Reviews

UNIQ3b1ef83ec736b33b-partinfo-00000000-QINU

No review score entered. 2012 Utah_State iGEM Team

The 2012 Utah_State iGEM Team has improved upon this part with BBa_K844000. BBa_K844000 improves on this part by removing the upstream Met (ATG) codon, extending the His repeat region to 10x (which improves binding affinity and purity of extracted protein product), and adding the double stop (TAATAA) downstream of the His tag to ensure protein termination, without the need for an additional cloning step for the stop codon. BBa_K844000 is also readily compatible with the Assembly Standard #23 (Silver Fusion)

UNIQ3b1ef83ec736b33b-partinfo-00000002-QINU