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Part:BBa_K900001:Design

Designed by: Austin Jones   Group: iGEM12_Berkeley   (2012-09-27)
Revision as of 22:31, 3 October 2012 by Austin.jones (Talk | contribs)

PAGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization for yeast and internal restriction site removal to be compatible with our Golden Gate cloning scheme.


Source

Synthesized in house and cloned into addgene plasmid l11911 Previously engineered version of RFP from Patterson and Lippincott-Schwartz, 2002.

References

Patterson, G. H. & Lippincott-Schwartz, J. A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells. Science 297, 1873–1877 (2002).