Part:BBa_K847000

''Deinococcus radiodurans'' DNA repair mechanism, Recombinase A

Deinococcus radiodurans is an extremely radiation-resistant bacterium: while about 10 Gy (absorbed radiation dose, Gray) can kill most vertebrates, D. radiodurans can withstand up to 12,000 Gy. Current literature supports this finding with D. radiodurans’ unique genetic makeup which allows it to better handle radiation exposure. The two main effects of radiation exposure to bacterial cells are DNA damage and the creation of toxic superoxide species (Daly 2009). Two DNA damage prevention and repair proteins in D. radiodurans have been shown to outperform analogs in less radiation-tolerant bacteria. The recombinational repair protein Recombinase A from D. radiodurans is much more effective at protecting DNA from damage than that from E. coli (Slade and Miroslav 2011).

Sources:

Daly, M.J. (2009) A new perspective on radiation resistance based on Deinococcus radiodurans. Nature Rev. Microbiol, 7: 237-245.

Slade, D. and Miroslav, R. (2011). Oxidative stress resistance in Deinococcus radiodurans. Microbiol. Mol. Biol. Rev., 75(1): 133-191.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 224
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 898
  
• 1000
  COMPATIBLE WITH RFC[1000]