This part contains a T7 promoter, lac operator, 6x his tag and the coding sequence for VioD and VioC (enzymes in the violacein production pathway).
Usage and Biology
A device was constructed that contained the two enzymes VioD and VioC which on their own do not produce a pigment.
However, when cells containing this construct were grown together with strains containing our VioABE construct BBa_K726016 , a purple pigment (violacein) was produced.
More information on this process can be found on the 2012 UCSF iGEM Violacein Results Page .
Sequence and Features
Assembly Compatibility:
10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2571 Illegal BamHI site found at 123
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1063 Illegal SapI.rc site found at 1138
