Coding
SAMT

Part:BBa_J45001:Experience

Designed by: Kate Broadbent   Group: iGEM06_MIT   (2006-06-07)
Revision as of 21:46, 8 June 2006 by Kate Broadbent (Talk | contribs) (→‎Enzyme Activity)

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Enzyme Activity

  1. E. coli-expressed SAMT protein catalyzes the formation of the volatile ester methyl salicylate from salicylic acid with a Km (SA) of 83 and a Km (SAM) of 3-4. It can also methylate benzoic acid to form methyl benzoate, but its K(m) value for benzoic acid is 1720 M (much larger). Kcat/Km for SA is 132 s-1 M-1, and activity with substrate benzoic acid is 45% of the activity with substrate salicylic acid. All other acids yield 0% activity.

UNIQ55a42332a8e21ab0-partinfo-00000000-QINU UNIQ55a42332a8e21ab0-partinfo-00000001-QINU