Coding
BSMT2

Part:BBa_J45007:Design

Designed by: Boyuan Zhu   Group: iGEM06_MIT   (2006-06-07)
Revision as of 21:38, 8 June 2006 by Boyuanzhu (Talk | contribs) (→‎Design Notes)


SAM:benzoic acid/salicylic acid carboxyl methyltransferase II; converts salicylic acid to methyl sal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 663
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 204


Design Notes

Just CDS taken from GenBank

Designed Forward Strand (1 and 2): 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG GAA GTT GTT GAA GTT -3'

  • LENGTH: 48
  • GC CONTENT: 45.8 %
  • MELT TEMP: 67.7 ºC


Designed Reverse Strand (phBSMT2): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAA C -3'

  • LENGTH: 52
  • GC CONTENT: 38.5 %
  • MELT TEMP: 65.0 ºC

Source

Species: Petunia x hybrida from GenBank (AY233466); (also available: A. thaliana and N. suaveolens)

References

    • <biblio>Underwood05 pmid=15849311</biblio>
    • <biblio>Negre03 pmid=14630969</biblio>