Part:BBa_J45001

SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winter

1. From snapdragon (A. Majus)
2. This carboxyl methyltransferase gene encodes an enzyme that catalyzes the transfer of the methyl group of SAM to the carboxyl group of salicylic acid to make the volatile ester methyl salicylate

Usage and Biology

1. The culture medium of the E. coli cells expressing SAMT contained methyl salicylate (2.1 μg/ml) when the growing medium was supplemented with 5 μg/ml salicylic acid, and methyl benzoate (0.86 μg/ml) when the growing medium was supplemented with 5 μg/ml benzoic acid.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 577
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 901
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 421
  Illegal NgoMIV site found at 425
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 471
  Illegal SapI.rc site found at 75
  

Functional Parameters

ec_num	none
kegg	none
protein	SAMT
swisspro	Q8H6N2