Part:BBa_K892404

HB36.5 A326E

A mutated HB36.5 flu binder aimed to increase binding to H2, a subtype of hemagglutinin on the surface of the Influenza virus. This mutant has a point mutation at residue 326 from Alanine to Glutamate

Usage and Biology

This part was tested by the [http://2012.igem.org/Team:Washington 2012 University of Washington] iGEM team to bind to various subtypes of Hemagglutinin in Group 1.To test BBa _K892401, it was inserted into a protein expression vector, pETCON. HB36.5_A326E was then produced and transformed into yeast as described in the [http://2012.igem.org/Team:Washington/Protocols/Yeast 2012 Team's Yeast Transformation Protocol]. The binding protein was then tested for activity against H2. For a detailed description of the methodology, please see the [http://2012.igem.org/Team:Washington/Protocols/Display 2012 UW iGEM Yeast Surface Display protocol]. The resulting data is shown below.

HB36.5 and its variants tested against hemagglutinin subtype 2. HB36.5 is shown to have very little activity in binding H2.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 107
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 107
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 289
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 107
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 107
  
• 1000
  COMPATIBLE WITH RFC[1000]