Coding

Part:BBa_K801040:Design

Designed by: Dong-Jiunn Jeffery TRUONG   Group: iGEM12_TU_Munich   (2012-09-21)
Revision as of 15:03, 3 October 2012 by Nadine1990 (Talk | contribs) (→‎Design Notes)

SV40NLS-PhyB-linker-Gal4DBD (part for GAL4 based light switchable promoter system)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 550
    Illegal BamHI site found at 632
    Illegal XhoI site found at 583
    Illegal XhoI site found at 602
    Illegal XhoI site found at 2737
    Illegal XhoI site found at 3071
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2122
    Illegal BsaI site found at 2528
    Illegal BsaI site found at 2990
    Illegal SapI site found at 799



Composite part of BBa_K801030, BBa_K801030, BBa_K365005, BBa_K801035


Keywords: GAl4, PIF3, phytochrom B, light switchable
Abbreviations:

  • UAS= upstream activity sequence

Design Notes

Related BioBrick: 

,BBa_K365005: Name_of_part
,BBa_K801035: Name_of_part


Cloning details:

  • Designed in RFC25

Quality control measures:

Backbone:

  • Backbone name: pSB1C3

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:


Organism:


References

Literature references:

Database references: